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On day 2, slices were washed 35 min in PBS 0.01 M at RT 4 mL/well and incubated in donkey anti-guinea pig-AF647 (1:500, Jackson ImmunoResearch) diluted in [PBS 0.01 M + 0.3% Triton]

What to eat: Continue with smaller portions but you can start reintroducing slightly more variety

Brush your teeth at least 30 minutes before taking drops, not immediately before

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Preliminary data suggest that GLP-1RAs may be effective in the treatment of hypothalamic obesity, and the presence of GLP-1R in various regions of the hypothalamus and in other regions of the brain explains the potential effectiveness of GLP-1RAs even in cases of hypothalamic damage [16]

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