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The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

Daniel Kelly, Jeffrey Martin, Steven Deeks, Timothy Henrich, Michael Peluso and Steven P

Hemolytic anemia may be more prevalent in patients with both MTHFR and B12 deficiency

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In the same 3 mL it is only ~3,333 mcg/mL (1 unit 33.3 mcg), so the same milligram doses need roughly double the units : 250 mcg 8 units and 500 mcg 15 units