The gonads were collected into a 1.5 ml microcentrifuge tube, fixed with 4% formaldehyde prepared in egg buffer for 20 min, and washed twice with 1 ml of 3% BSA in PBS
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Influence of glucagon on GFR and on urea and electrolyte excretion: direct and indirect effects
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Check for co-factors like Vitamin C
Try to pair a decent diet with regular movement, but not extreme overtraining, which can cause hormone imbalances