Animal model and experimental protocol Male C57BL/6 mice (8 weeks old) weighing 25 0.5 g were obtained from the Jackson Laboratory (Bar Harbor, ME)
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Digestive bitters (bitter herbs) prior to meals is advised
For molecular analysis, immunofluorescence, and immunohistochemistry techniques were extensively used on human testis, mouse testis, and TM3 cells to visualize the expression and subcellular localization of key proteins