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Temperature excursions above 25C for more than 24 hours degrade methylcobalamin and can oxidise methionine

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After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]

625 - 638 Copyright The Authors 2019 Carnitine is a vitamin-like substance that plays a vital role in the -oxidation of long-chain fatty acids (FA) to produce energy by transporting them from the cytosol to the mitochondrial matrix, via carnitine palmitoyltransferase 1 (CPT1) ( Reference Bremer 1 , Reference Zammit, Ramsay and Bonomini 2 )

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