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Ethics declarations Ethics approval and consent to participate The whole protocol was approved by the Research Ethics Committee of Mashhad University of Medical Sciences (registration code: IR.MUMS.fm.REC.1396.671), and written informed consent was obtained from all of the patients or their families directly

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2.6 Measurement of inflammatory cytokines and antioxidant indicators Levels of cytokines (TNF-, IL- 1 , IL-4, IL-6, IL-10, TGF-) and antioxidant indicators (T-AOC, T-SOD, GSH-Px, MDA) in serum and ileal mucosa were measured using ELISA kits (FANKEW, Shanghai Kexing Trading Co., Ltd., China)

This is why the refrigerate always and 28-day puncture rules are much more strictly enforced for compounded products