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The best method of preparing them is raw or lightly steamed because heat can damage the amount of sulfur compounds available

At the end of the experiment, RNA was extracted from the cells, and RT-qPCR analysis of gene expression was carried out using specific primers against rat fibroblast-specific protein-1 (FSP1), keratin-19 (KRT19), aggrecan, and type I and type II collagen

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These entities are essentially comprised of solid neoplasms, haematological malignancies and liver and kidney diseases

J Clin Invest 119(4):747754

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