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After harvesting and washing with 1xDPBS without CA and Mg (Corning, 21-031-CV), RIPA buffer (Thermofisher, #89900) supplemented with a protease inhibitor (cOmplete, Roche, #11697498001) was added and the lysate was incubated for 30 min at 4 C

Average decreases in folic acid concentrations of 36% (dark) and 39% (light) after 6 months and 47% (dark) and 50% (light) after 12 months of storage were observed 487

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Available from: Simon L et al (2014) Paternal influence of sperm DNA integrity on early embryonic development

also called erythroblastosis fetalis), the fetus has inherited paternal blood group factors (Rh and/or ABO) that differ from those of the mother-to-be [170, 171]