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Acetonitrile (HPLC-grade), triethylamine, formic acid, trichloroacetic acid (TCA), 2-mercaptoethanol, and N -ethylmaleimide (NEM) were obtained from Nacalai Tesque (Kyoto, Japan)

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Fragmented ECM components (e.g., platelet-derived proteins and proteoglycans) generated during tissue remodeling are recognized by immune cells as DAMPs, further amplifying inflammatory and fibrotic signaling via TLR2/4 [318, 319]

Injecting through wet alcohol causes stinging