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ISH provides high cellular resolution, especially since the development of the RNAscope protocol (82) that has increased sensitivity and specificity, but this technique only measures mRNA and not receptor protein expression

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MGF is not subject to IGFBP sequestration (its E-domain does not bind any known IGFBP) and can therefore be used at defined concentrations in any media composition without the confound of IGFBP-mediated concentration uncertainty

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