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J., Larson, S

2013;8(9):e74558

The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) ( Recently, we developed a gene transfer-positive cell sorting system using membrane anchoring Twin-Strep-Tag ( Glutathione-S transferase (GST) is a multifunction enzyme that catalyzes the conjugation of glutathione to electrophilic compounds ( Results Design the membrane anchoring glutathione S-transferase fusion maker We use membrane anchoring GST molecule as the affinity tag to allow the transfection-positive cells to be isolated using GSH beads (Figure 1)

Suitable for individuals with cardiovascular concerns

Liu W-T, Jing Y-Y, Yu G-F, Chen H, Han Z-P, Yu D-D, Fan Q-M, Ye F, Li R, Gao L, et al

Its pretty much the current IT- preservative