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You dont throw up physically, but youve got that feeling and I was about two, three weeks where I felt nauseous the whole time

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Here is a quick storage guide Before reconstitution (powder) Storage : Refrigerate at 2-8C (36-46F) Shelf life : 2-3 years when properly stored Freezer : Can freeze for longer storage (3-5 years) Room temperature : Survives short periods (days to weeks) but refrigeration preferred After reconstitution (liquid) Storage : Refrigerate immediately at 2-8C Shelf life : 28 days with bacteriostatic water Freezer : Not recommended (can damage peptide structure) Room temperature : Begins degrading within hours, use within days max Key rules Always refrigerate reconstituted peptides Use bacteriostatic water (not sterile water) Mark reconstitution date on vial Protect from light (store in box) Use within 28 days after reconstitution Never shake vials vigorously Understanding peptide stability Why peptides degrade Chemical breakdown : Peptide bonds can break down over time, especially in liquid form

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

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