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ce , TA cloning-based sequencing verifying Q27N ( c , 41%), Q27E ( d , 43%), and Q27L ( e , 43%) mutations

Tumor necrosis Factor--Induced Protein-8-like 2 transfected adipose-derived stem cells regulated the dysfunction of monocrotaline pyrrole-induced pulmonary arterial smooth muscle cells and pulmonary arterial endothelial cells

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Cells were washed three times with 1 ml PBS and lysed with 200 l 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 C for 5 min, followed by the addition of 800 l PBS

Reconstituted stability: 14 days at 2-8C in copper-free polypropylene containers, protected from light

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