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Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature

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Because Accutane is a complex medication with many side effects, it should not be taken without the medical advice of a board-certified physician, such as Dr

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The mean of the correlation coefficients of the standard curves were all above 0.98

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