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Cells were washed three times with ice-cold PBS and lysed in PBS with 1% Triton X-100, 20 mM Tris-HCl

The article also discusses their specific mechanisms and potential as future therapeutic treatments.

This may further help decipher their physiological significance, e.g., when the data on phenotypes of the mutated isoenzymes in humans may appear

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The Nrf2-ARE signaling pathway activates and enhances the expression of downstream antioxidant enzymes, serving as a critical cellular defense mechanism against oxidative stress (236)

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