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Before NMR analysis, the sample was reconstituted in 50 l of precooled 4 C methanol, centrifuged at 18,500 g for 10 minutes at 4 C and the resulting supernatant was transferred into a precooled 2 ml Eppendorf and then stored at -80 C [25]

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In addition, circular RNA inhibits ferroptosis by inhibiting miR-874-3p and regulating gene expression of related transmembrane proteins such as glycerophosphodiester phosphodiesterase 2 [144]