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The Fe 2+ absorbed and from recycling is oxidized to Fe 3+ form by two copper-containing oxidases, ceruloplasmin (CP) or the homolog hephaestin [32, 33], which is essential for efficient binding to transferrin

Nestin is expressed in many other tissues, including multiple cells during embryonic development and neural stem cells (33)

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These points come from official label language and vial-handling guidance, not from casual forum summaries

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