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Glucoraphanin is the precursor to sulforaphane, and the conversion is supported by myrosinase

Altogether 300 samples were collected, but the consent for one sample was retracted resulted in 299 reference samples

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RT-qPCR was performed using TB Green Premix Ex Taq II (Takara Bio, Tokyo, Japan) and the QuantStudio 7 Flex RealTime PCR system (Applied Biosystems, USA)

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