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Ten microliters of SYBR Green PCR Master Mix (Bioline, UK), 1 l of cDNA template, 2 l (10 pmol/l) of gene primer, and 7 l of nuclease-free water made up the 20 l reaction volume

In rare cases, inflammation of the gallbladder, liver, or pancreas resulting from gallstones can become life-threatening (Zakko 2018

Widely used across Asia, it is trusted for its antioxidant benefits and skin-enhancing properties

Abdominal cramping: Usually temporary and may be mitigated by taking the supplement with a light meal

5 l of 10% trifluoroacetic acid (TFA, cas 76-05-1, Sigma Aldrich, #302031) was added to each sample to quench the reaction

C., Liang, L., Hong, F., Fu, J