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(2) Mechanistic superiority: Dual inhibition of TBK1 and IKK in eWAT restores AMPK-Thr172 phosphorylation, enhancing mitochondrial biogenesis (via UCP1 upregulation), catecholamine sensitivity (via cAMP upregulation and cAMP-mediated -adrenergic signal transduction) [201, 202]

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The protein was then incubated overnight with TEV protease on the column to remove the C-terminal 2MBP-tag in the buffer above at 4 C

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Therefore, balanced nutrition is essential for maintaining metabolic health