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Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates

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In the current HepG2 cell model, in which prolonged exposure is not feasible, the use of relatively high QI concentrations in a short timeframe allows for the identification of underlying mechanisms and pathways, as has been done previously in similar studies with hepatic toxicants (Tafazoli and OBrien, 2009

Given the high conservation of the decoding center across species 36 , we used a combination of tRNA and ribosomes from distinct sources to prepare cryo grids P

Injections bypass the gut and correct deficiency