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While this effect typically diminishes as the body adjusts, it provides indirect evidence that the nitric oxide pathways are responding to the peptide

8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

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Since this discovery, GHK-Cu has been widely used in topical anti-aging skincare products and hair regrowth formulations