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On the other hand, insulin secretion was primarily stimulated by high glucose and amplificated by Gs-coupled receptors such ss GLP-1-stimulated GLP-1 receptor in GLP-1R-MIN-6 cells

5-bromo-4-chloro-3-indolylphosphate (BCIP)-nitroblue tetrazolium chromogen (substrate) reacted with the streptavidin-alkaline phosphate complex, forming a purple-brown precipitate, resulting in a visible banding pattern on the strip

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Step 2: Store Correctly