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Follow a cycle consisting of three weeks on and one week off

At 48 h after plating, the medium was replaced with DMEM supplemented with 5% horse serum (Sigma), 20 mM D-glucose and, on day 4, cytosine arabinoside (10 M) to prevent non-neuronal proliferation 33

Those two facts fit together, although it is still worth remembering that better average performance in trials does not guarantee that every patient will respond better to tirzepatide individually