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To the rest of the sample, 2.5 l of 100 mM of the tris (2-carboxyethyl) phosphine (TCEP) in 50 mM ABC (pH = 7.5) was added, followed by 30 min incubation at 37 C with shaking

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The assay includes a control test tube to ensure the accuracy of the GPx activity measurements and address potential interferences from other thiols and sample components