For reconstitution, the standard research approach for a lyophilized GHK-Cu vial involves bacteriostatic water added slowly along the vial wall (never injected directly onto the powder) and gently rolled never shaken until fully dissolved
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Western blot Cells were lysed in 1 Laemmli buffer and centrifuged at 10,000 g for 2 min through a Nucleo Spin filter (Machery Nagel)
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