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We utilized serum-free medium to avoid any interference of growth factors and proteins that are present in serum and which might affect the adhesive properties of Jurkat cells in our experiments

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The answer is about coverage and contact time

This is a general principle that applies to therapeutic peptides, though specific data on expired semaglutide reactions is limited

C., Bustamante, S., Najib, N., Diwan, A., Jayasena, T., Chowdhury, N