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Sterile ELISPOT plates with a PVDF membrane (Millipore Corp, Bedford, MA, USA) were coated with anti-human IgG (15 g/mL, Mabtech, Nacka Strand, Sweden) overnight at 4C

The Oxidative Stress Profile measures lipid peroxidation, DNA oxidation, and glutathione status, key markers for evaluating the downstream effects of chronic inflammation

The naming distinction is important for insurance and medical records