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After 1 mM CaCl 2 was added for Tse3-Tsi3 protein interaction, the supernatant was then loaded to the CNBr-activated Sepharose resin (GE Healthcare) conjugated with Tse3 protein by gravity flow

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Cardiovascular outcomes and other key takeaways Overall, the authors found that all six OMMs were associated with significant improvements in weight loss compared to a placebo

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