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One milliliter of sample was withdrawn at each time point (0.5, 1, 2, 4, 8, 12, 24, 36, and 48 h) from the Franz cell receptor chamber and refilled with an equal quantity of fresh PBS (pH 7.4)

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Moreover, lactate is a critical energy substrate for astrocytes and neurons, and its dysregulated metabolism in ME/CFS and FM patients may reflect an impaired ability to use lactate effectively for energy (Blanger et al., 2011)

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