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According to Zhang et al., sevoflurane and isoflurane might induce neuroinflammation and cognitive impairment by increasing the level of interleukin-6 (IL-6) via nuclear factor-kappa B (NF-B) signaling, which is associated with M1 activation (Zhang et al., 2013)

doi: 10.1074/jbc.274.20.13908 41 IslingerMLiKWSeitzJVlklALersGH

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10.1093/carcin/bgv009 127 ZhangP.LiS.ChenZ.LuY.ZhangH

To recover both necrotic and apoptotic cells, the 96-well plates were centrifuged at 400 g for 10 min and supernatant was discarded

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