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The collected cells were suspended in 300 L ice-cold TE buffer (100 mM TrisHCl (pH 8.0), 2 mM ethylenediaminetetraacetic acid) and disrupted by a cell disruptor (Shake Master NEO, Bio Medical Sciences, Tokyo, Japan) with zirconia beads at 1500 rpm for 10 min

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Moreover, AtGLR2.5 and AtGLR3.6 have been identified as additional contributors to Ca 2+ -dependent stress signalling (Gong et al