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Gels were subsequently transferred into a 0.45-m nitrocellulose membrane, blocked with 5% milk in TBST buffer for 1 h, and incubated overnight at 4C with primary antibodies (1:1,000) against carnitine palmitoyltransferase 2 (Millipore AB585), carnitine-acylcarnitine translocase (Proteintech 19363-1-AP), and heat shock protein 70 (Santa Cruz Antibodies 7298) as a loading control

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Been using for shoulder pain for about 3 weeks