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Mice and rats were anesthetized with subcutaneous ketamine/xylazine, the corneal surface was numbed with 0.5% proparacaine drops, and pupils were dilated with sequential drops of 0.5% tropicamide and 2.5% phenylephrine HCl

Subsequently, prostaglandin and thromboxane generated by cyclooxygenase as well as leukotrienes generated by lipoxygenase from arachidonate (20:4n-6) function as lipid mediators

Follicles with a large antrum, and clearly distinguishable corona radiata and cumulus oophorus cell clusters were classified as antral follicles

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