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Standard Rest Periods: After 10-day cycle: 20-30 days rest After 20-day cycle: 40-60 days rest After extended cycles: 90+ days rest During rest periods, researchers can analyze collected data, plan protocol modifications, and prepare for subsequent cycles

Santhekadur PK, Kumar DP, Sanyal AJ (2018) Preclinical models of non-alcoholic fatty liver disease

Monitor micronutrient status Smaller meal volumes can reduce intake of key vitamins and minerals

Increased intestinal permeability and tight junction alterations in nonalcoholic fatty liver disease (in Eng)