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This method employs Annexin V conjugated to FITC to detect early apoptosis and propidium iodide (PI) to detect late apoptosis in a 6-well plate were grown to a confluency of 70% in the different groups and the supernatant was collected

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Moreover, there are some limitations due to the use of a pharmacy-based database: first, patients who purchased prescriptions outside the pharmacies included in the database were not trackable