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For EcGGT, truncation beyond 17 amino acids was found detrimental for maturation, indicating the crucial role of the last nine amino acids of the signal peptide in functional expression of the enzyme (Lo et al., 2008), while in BlGGT, all the truncated mutants except the native enzyme resulted in partial or complete loss of processing, emphasizing again on the importance of signal peptide in the enzymes folding and maturation (Lin et al., 2008)

10.7150/thno.22679 87 ZhouR.WangH.YangY.ZhangC.DongX.DuJ.et al (2019)

Summary: Adults with B12 deficiency and nerve pain typically require 1 mg hydroxocobalamin injections intramuscularly on alternate days until symptoms improve, followed by maintenance injections every two months

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