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In very few cases (e.g., enzyme-linked immunosorbent assay, ELISA

pharmacological inhibition of CYP24A1 reduces the expression of fibrosis-associated markers [110]

Compared to acute wounds, the number of M1 macrophages has increased by 2-3 fold, while M2 macrophages have decreased by approximately 50%60%, as determined by flow cytometry and immunohistochemical analysis of wound biopsies (Shao et al., 2025

However, HRT is not always the sole solution

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Physiological stimuli such as shear stress or G protein-coupled receptor (GPCR) agonists like bradykinin promote Ca 2+ entry, leading to dissociation of the Cav-1-eNOS complex and allowing eNOS to be activated via phosphorylation by kinases such as Akt (Feron et al., 1998)