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Following the indicated experimental design, 5 10 4 FACS-sorted ILC2s were plated on a Seahorse XFp PDL-coated cell culture miniplate in triplicates in FBS/Phenol red free Seahorse media supplemented with 1 mM pyruvate, 2 mM glutamine, and 10 mM glucose

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both are included to support a calm, comfortable, well-supported skin environment around treatment

Here is how to address common issues that arise during GHK-Cu protocols