A similar protocol was followed for glucose-stimulated GLP-1 secretion in TC1-6 cells, except for incubations in 5 mM and 25 mM glucose, to conform with established methods for these cells (37, 38)
doi: 10.1016/j.neuron.2014.07.027 [DOI] [PubMed] [Google Scholar] 18
The FDA defines a drug shortage as a period when the demand or projected demand for a medically necessary drug in the United States exceeds its supply
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However, the substitution of residues in the N-terminal half of GLP-1 for those of glucagon had much smaller effects (Hjorth et al ., 1994