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chelation of the copper site by the thiol group, interference with the cellular transfer of tyrosinase to premelanosomes, which is the site of melanin synthesis and indirect inhibition of tyrosinase via its antioxidant effect as the free radical scavenging effect of it blocks the induction of tyrosinase activity caused by peroxides

Reverse transcription of total mRNA were performed at 1 g of total mRNA in 25 l final volume using random primers and avian myeloblastosis virus reverse transcriptase

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