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Cells were then treated with 10 M of compound 6a or DMSO, with 0.1% DMSO adjusted for both conditions in fresh medium for 8 h incubated in a humidified atmosphere at 37 C and 5% CO 2

7.2.1 Priorities for clinical studies Large-scale, long-duration randomized controlled trials (RCTs) are urgently needed to evaluate how emerging combination therapies impact hard renal endpoints, including serum creatinine doubling, progression to kidney failure, and renal death

The more general anti-aging peptide literature identified 199 peptides investigated for skin aging, illustrating where research effort concentrated, while fertility-specific peptides were still fewer and less well-studied