Materials and Methods Cell Culture HeLa cells were cultured in DMEM +FBS [DMEM without pyruvate and glucose (ThermoFisher, 11966025) supplemented with 25 mM glucose, 10% non-dialyzed FBS (Sigma, F2442) and 1% penicillin/streptomycin] at 37C in 5% CO 2
They measured subjective sleep quality more than objective sleep quality They subjectively estimated tiredness Sleep structure was not fully characterized by polysomnography in all studies Data analysis suggested some observed changes were due to issues with the placebo group What is promising about these findings is that statistically significant effects were found in such small sample sizes
doi:10.1186/s12916-016-0657-8
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Salmon AB, Richardson A, Perez VI: Update on the oxidative stress theory of aging: does oxidative stress play a role in aging or healthy aging
Stress and Sleep Deprivation: Chronic stress keeps the body in a state of high alert, increasing the production of free radicals and putting a heavy load on your antioxidant defenses
