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Total proteins of each sample were separated on a 10% SDS-PAGE gel and electrophoretically transferred onto a PVDF membrane (EMD Millipore, Billerica, MA, USA)

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The study reportedly states that dual-loaded Chitosan nanoparticles demonstrated greater anti-proliferative activity against a breast cancer cell line (MCF-7) than doxorubicin-loaded Chitosan

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