Grx activity was determined by monitoring the consumption of NADPH at 340 nm during the GSH-dependent reduction of 8 mM -hydroxyethyl disulfide (HED) using 1 mM GSH coupled with of 0.6 units of yeast GR (Sigma) [20]
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It is an important polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water
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