Because of its ease from the viewpoint of both the theory (flexible parts are digested, rigid parts are not) and practice (mix a query protein with a limited quantity of a protease, wait for a limited time, and separate the components of the resulting mixture) and usefulness of the generated information, this approach has a long history of useful utilization in protein research
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The study of Seyedinia et al., was conducted on female rats that were injected with VPA on the 12.5th day of pregnancy to induce an experimental model of autism
The remaining worms were transferred to fresh plates according to their respective conditions: (1) fresh food plates, (2) empty plates for fasting, or (3) empty plates for continuous fasting