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Their broad gene-regulatory capacity continues to make them a compelling subject for researchers aiming to uncover the full spectrum of their therapeutic and cosmetic research applications

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The cDNA was amplified in the Opticon Continuous Fluorescent Detector (MJ Research, Waltham, MA, USA) using IQTM SYBR Green supermix (Bio-Rad) and sequence specific primers

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We further mutated individual residues at the extracellular end of TM7 from E297 to I300 to proline and measured ghrelin-induced GHSR activation

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