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Cells were incubated at room temperature for a further 2 h and stimulated with 10 nM GLP-1 or GIP in HEPES-buffered saline (HBSS) containing 107 mM NaCl, 6 mM KCl, 1.2 mM MgSO 4 , 2 mM CaCl 2 , 1.2 mM KH 2 PO 4 , 20 mM HEPES, and 5.5 or 16.7 mM glucose at pH 7.4