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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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Cyanocobalamin is the FDA-approved standard for treating deficiency and is praised for its stability

In addition to ferrous ion, many metal ions including Cu (I), Cr (II), and Co (II) were found to have the oxidative features of the Fenton reagent

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