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After 3 h for cell attachment, medium was exchanged to Sera Plus-free medium and cells were allowed to adjust for 16 h before treatment

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Ding, D., Wang, M., Wu, J

Interestingly, GSTO1 was identified as one of the potential cellular targets of piperlongumine, an electrophilic compound exerting selective cytotoxicity against cancer cells through an oxidative cell death mechanism (Supplementary Table 25) 37

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